The LIPH4SAS (“Livestock Phenotyping for Sustainable Agroecological Systems”) research infrastructure is dedicated to phenotyping livestock and enables experiments, phenotyping and biological sampling for the animal-research community, with a view to promote the transition to more sustainable agricultural and food systems based on agroecological principles. LIPH4SAS consists of eight experimental units (four for ruminants, two for pigs and two for trout). It also includes a platform for studying livestock physiology in depth and a group of engineers responsible for managing data and developing phenotyping tools. It provides skills and tools for detailed and multi-scale phenotyping (e.g., surgery, in vivo and ex vivo imaging, respiration chambers) and horizontal phenotyping of large groups of animals (e.g., slaughterhouses; equipment for measuring growth, feed intake, feed-use efficiency, body composition, behaviour, health, product quality, and greenhouse gas emissions). LIPH4SAS aims to provide a global service that promotes interaction between scientific communities, harmonizes practices to ensure the highest levels of expertise and ethics possible in animal experimentation and welfare, encourages innovation in animal science and facilitates accessibility to the findability, accessibility, interoperability and reuse (FAIR) of data.